Correction: The Crystal Structure and Small-Angle X-Ray Analysis of CsdL/TcdA Reveal a New tRNA Binding Motif in the MoeB/E1 Superfamily
نویسندگان
چکیده
There are multiple errors in Table 1. Please see the corrected Table 1 here. There is an error in the last sentence of the “TcdA crystallization, structure determination and refinement” subsection of the Materials and Methods. The correct sentence is: A data set for the AMP complex was collected to 1.80-Å resolution at the PROXIMA 2A beamline (Synchrotron SOLEIL, Paris, France). All the data sets were integrated with XDS [26] and scaled with Aimless [27] from the CCP4 suite of programs [28] (Table 1). There are errors in the fifth and sixth sentences of the “Crystal structure of TcdA” subsection of the Results and Discussion. The correct sentences are: To shed light onto the structural basis for the tRNA binding and ctA synthetic properties of TcdA-ATP, we determined the crystal structure of E. coli TcdA (Table 1 and Fig 2) loaded with ATP to 1.77 Å resolution (R/ Rfree values of 0.141/0.183) (Fig 3A) and AMP to 1.80 Å resolution (R/Rfreevalues of 0.139/ 0.176) (Fig 3B). The asymmetric unit contained four TcdA chains arranged in two independent dimers, with a solvent content of 39%.
منابع مشابه
The Crystal Structure and Small-Angle X-Ray Analysis of CsdL/TcdA Reveal a New tRNA Binding Motif in the MoeB/E1 Superfamily
Cyclic N6-threonylcarbamoyladenosine ('cyclic t6A', ct(6)A) is a non-thiolated hypermodification found in transfer RNAs (tRNAs) in bacteria, protists, fungi and plants. In bacteria and yeast cells ct(6)A has been shown to enhance translation fidelity and efficiency of ANN codons by improving the faithful discrimination of aminoacylated tRNAs by the ribosome. To further the understanding of ct(6...
متن کاملSynthesis and crystal structure of Schiff-base compound (E)-4-methoxy-N- (4-hydroxybenzylidene) aniline
New Schiff-base compound (E)-4-methoxy-N-(4-hydroxybenzylidene) aniline has been synthesized from the reaction of 4-hydroxybenzaldehyde with 4-methoxyaniline in methanol at 50ºC, and has been characterized by using elemental analysis and FT-IR spectroscopy. The crystal structure of the title compound has been determined by single crystal X-ray diffraction study. The title compound crystallizes ...
متن کاملIdentified Hybrid tRNA Structure Genes in Archaeal Genome
Background: In Archaea, previous studies have revealed the presence of multiple intron-containing tRNAs and split tRNAs. The full unexpurgated analysis of archaeal tRNA genes remains a challenging task in the field of bioinformatics, because of the presence of various types of hidden tRNA genes in archaea. Here, we suggested a computational method that searched for widely separ...
متن کاملOne-dimensional Uranium(VI) Coordination Polymer Complex Containing Dimethyl and Trimethyl Phosphate Ligands: Synthesis, Spectroscopic Characterization, Thermal Analyses, and Crystal Structure
A new one-dimensional uranium(VI) coordination polymer, [UO2(μ-DMP)2(TMP)]n (1) (DMP is dimethyl phosphate and TMP is trimethyl phosphate), was prepared from the reaction of UO2(NO3)2.6H2O and TMP in in THF (THF is tetrahydrofuran) as a solvent. Suitable crystals of this complex for crystal structure determination were obtained by slow evaporation of the produced yellow solution at room tempera...
متن کاملSYNTHESIS, X-RAY CRYSTAL STRUCTURE AND IR STUDY OF NEW TRINUCLEAR, BASE CARBOXSYLATE COMPLEX OF [Cu3O(OAc)6(H2O)3]Cl.CH3OH.6H2O
The synthesis of [Cu3O(OAc)6(H2O)3]Cl.CH3OH.6H2O is described. The X-ray crystallographic study of thecomplex revealed an isosceles triangle of copper atoms with a triply bridging oxo atom nearly in the plane of thetriangle. The coordination sphere around each metal center is close to distorted octahedral and the central{Cu3(μ3-O)} core is planar. Every two copper atoms were connected to each o...
متن کامل